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Bioavailability and Conversion: Methylcobalamin is the active form of vitamin B12, meaning it does not require conversion by the body to be utilized
cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)
This emphasizes the significant role of vitamin B12 in DNA synthesis, myelin sheath maintenance, and cell metabolism, underscoring its essential contribution to promoting good health
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