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To determine oxidative stress on the cells dosed with menadione, we monitored GSSG levels (Figure 3, Panel B, and Figure 4, Panel B)

To the 10.000 rpm supernatant containing microsomes and cytosol fractions, 0.2 volume of 0.1 M calcium chloride solution was added, and further centrifuged at 10,000 rpm for 10 minutes to precipitate the microsomes