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LC-MS analysis was done on a Waters SYNAPT quadrupole/time-of-flight mass spectrometer operated in positive ion electrospray mode

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After washing, membranes were incubated with goat antirabbit IR Dye 680LT (#926-68021, 1:5000, Li-COR Biosciences, Lincoln, NE, USA) or goat antimouse IR Dye 800CW (#926-32210, 1:5000, Li-COR Biosciences) fluorescent secondary antibodies secondary antibodies and visualized using an Odyssey infrared imaging system (Li-COR Biosciences)