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Exposure of pregnant rats to FBs impairs bone metabolism of the offspring via alteration of the osteoprotegerin (OPG)-to-RANL (receptor activator of nuclear factor B ligand), possibly driven by immunological alterations (153), and by affecting the expression of remodeling enzymes [MMPs and their tissue inhibitors (TIMPs)] and angiogenic factors [vascular endothelial growth factor (VEGF)] (154)

After centrifugation, the supernatant was carefully transferred to a 10 mL graduated cylinder, and the remaining volume (V) was recorded