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Oil Red Stain A and B were mixed and added to the culture plate (Beyotime, China) Staining was conducted for 30 min

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Relative gene expression The quantitative expression analysis of UCP1, UCP2 (uncoupling proteins), NAMPT (nicotinamide phosphoribosyltransferase), and FNDC5 (fibronectin type III domain-containing protein 5) was performed by real-time PCR using 18S rRNA ( Hs03003631_g1 ) ( Applied Biosystems ) as the reference gene

Helps reduce inflammation and recovery time, making it ideal for injury support and physical restoration