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The fluorescent spot test is the simplest, most reliable, and most sensitive of the screening methods

It examines all parameters for inflammation, blood sugar levels, insulin levels, immune system function, thyroid function, parathyroid hormone levels, mineral and electrolyte balance, zinc and copper ratio, vitamin A and D levels, folate, and vitamin B12 levels, a complete metabolic panel, complete blood count, liver function, kidney function, urinary tract health, nutrient deficiencies, and more

Then, 5 protein loading buffer containing DTT was added to the mixture and vortexed for 30 s to mix well, and it was heated at 100 C for 5 min

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