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Liver paraffin sections (5 mm) were prepared and stained with hematoxylin and eosin (H&E) by the Department of Pathology at the University of Colorado Denver using standard procedures

The four-pathway model therefore targets appetite (GLP-1 and amylin through separate neuronal circuits), fat metabolism (GIP and glucagon), energy expenditure (glucagon primarily), and glucose homeostasis (GLP-1, GIP, and amylin all contribute through different mechanisms)