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No invasive techniques are used, and no anesthesia is required
Additionally, those with liver or kidney disorders might find the increased metabolic activity induced by these injections to be problematic, as these organs are crucial in processing the substances in the injections
Spacing them by several hours eliminates this minor concern
Freeze/thaw cycles degrade peptides, so aliquot the reconstituted solution if you plan to run assays over several weeks
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