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10,11 Curcumin Preclinical and in vitro data suggest that curcumin can inhibit DPP-4 activity and enhance glycemic control in diabetic animal models, potentially prolonging the active life of GLP-1, although this has not been confirmed in human mechanistic studies

In brief, total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA, USA), quantified and purified using a Nanodrop2000 (Thermo, USA) and constructed using the Illumina TruSeqTM RNA Sample Prep Kit method

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For researchers in younger demographics, the same protocols apply, though the necessity of telomerase activation in individuals with adequate telomere length remains an open question