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Human TRPV1 (hTRPV1)-expressing CHO K1 cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% FBS, 1% penicillin/streptomycin, and 800 g/mL geneticin in an incubator with 5% CO 2 at 37 C
There are two 2HG enantiomers: D-2-hydroxyglutarate (D2HG) and L-2-hydroxyglutarate (L2HG), with the former predominantly generated from IDH1 mutants and PHGDH while L2HG is primarily generated from the promiscuous activity of LDHA and MDH1/2 under hypoxic or acidic conditions and converted back to 2OG by L2HGDH
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