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Cooled the mixture to 25-30C and stirred 10 hr at same temperature
Within experimental models, it is commonly investigated for peptide-mediated signalling dynamics and molecular interaction mechanisms
Modifies protein structure and function, affecting signalling pathways
These results were replicated by an immuno-EM approach in primary mouse islets transduced with a SNAP/FLAG-hGLP-1R-expressing adenovirus, by labelling cell surface SNAP/FLAG-hGLP-1R in intact living islets with membrane-impermeable SNAP-biotin plus streptavidin conjugated to 5-nm gold particles prior to exendin-4 stimulation and TEM processing
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