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Rather than replacing natural processes, the emphasis has shifted toward supporting the bodys own anabolic and recovery mechanisms through evidence-aligned formulation strategies

The sample size determination was based on previous similar experimental studies investigating ischemia-reperfusion injury, ensuring sufficient statistical power to detect intergroup differences in both biochemical and histological evaluations

Results from IHC showed GSK-3 inhibitor effectively reduced GSK-3 activity up to 61.3% with the addition of FUS-BBB opening and confirming the proposed therapeutic route