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For MST analysis, Syn variants, Syn WT -141C, Syn S129E -141C, and Syn 1103 -104C with a single cysteine residue near the C-terminus end were labeled using the Monolith NT Protein labeling Kit RED-MALEIMIDE (NanoTemper Technologies)

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In contrast, the KNN classifier achieved a precision of 87.23%, a recall of 86.43%, an F1-score of 85.71%, a G-measure of 86.26%, and an accuracy of 86.56% improvement