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50 mg vial reconstitution math BAC water added 3.0 mL Final concentration 16.7 mg/mL Research amount 1 mg Draw volume 0.06 mL U-100 syringe units 6 units BAC water added 3.0 mL Final concentration 16.7 mg/mL Research amount 2.5 mg Draw volume 0.15 mL U-100 syringe units 15 units BAC water added 3.0 mL Final concentration 16.7 mg/mL Research amount 5 mg Draw volume 0.30 mL U-100 syringe units 30 units BAC water added 3.0 mL Final concentration 16.7 mg/mL Research amount 10 mg Draw volume 0.60 mL U-100 syringe units 60 units The values are rounded

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A critical component was the establishment of an in vitro co-culture system using 12-well plates and 0.4 m pore size transwell inserts