rbc glutathione test functional medicine Lab Testing rbc glutathione test functional medicine

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Samples were reconstituted in 3060 l 50% acetonitrile, followed by vortexing for 30 sec, and then centrifuged at 10,000 rpm for 10 min at 4 C

Briefly, each primary antibody was incubated for 12 h in a humidified chamber at 37 C, followed by detection using the HRP-conjugated secondary antibody and TSA-dendron-fluorophores (Histova, NEON 7-color IHC Kit for cryosection, 1:100), after which the primary and secondary antibodies were thoroughly eluted in stripping solution (Histova, Abcracker) for 60 min at 37 C

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