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The external validity of previous findings from rodent experiments is thus limited

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213,216 NAD + -dependent modification of metabolic enzymes Beyond serving as a hydride-donating coenzyme for metabolism, NADH/NAD + also acts as co-substrate for the sirtuins-mediated post-translational modification of metabolic enzymes including acetylation, ADP-ribosylation, succinylation and malonylation