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All haematoxylin and eosin and immunohistochemistry staining was performed on 5 m formalin-fixed, paraffin-embedded sections that had previously been heated at 55 C for 45 min

43, 335367.10.1146/annurev-genet-102108-134201 42 MittovaV.TheodoulouF

Ethics declarations Funding This work was supported by grants from the NSFC-CIHR (81261120565 to JW), the Program for Changjiang Scholars and Innovative Research Team in University (82000-18811100 to JW), the Program for 973 project (2012CB517506 to JW), the National Natural Science Foundation of China Grant Award (81300705 to FX) and the Fundamental Research Funds for the Central Universities (12ykpy41 to FX)

By week 12, all three receptor pathways are fully engaged and working together, which is why the transition from month 3 to month 6 often shows accelerated weight loss compared to the initial adaptation period