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HUBERMAN: In order to find out the lethal dose of something, as you can imagine, unfortunately, the way these studies are done is they give animals more and more, that is higher and higher doses of a given compound, find out at what point about 50% of the population of those animals starts to die, and then that's the so-called LD50, or at least that's one crude way of describing it

After initial analyses, the remaining cells were centrifuged and stored as pellets at -80C for further investigations

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10.1038/s41423-021-00807-4 Cell Mol Immunol