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Raised interleukin-6 levels in obese patients

Serious adverse effects appear rare but remain possible until more comprehensive long-term studies are completed

Subsequently, samples were blocked and permeabilized with 5% bovine serum albumin (Solarbio) and 0.25% Triton X-100 (Beyotime) for 1.5 h and then incubated overnight at 4 with primary antibodies: Nestin (R&D systems, MAB2736), SOX2 (Abcam, ab93689), Tuj-1 (Abcam, ab18207), GFAP (Cell Signaling Technology, 3670), Olig2 (R&D systems, AF2418) and GPX4 (Abcam, ab125066)