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This intermittent use pattern, while not extensively studied, may provide a practical approach for individuals who cannot afford or tolerate continuous long term treatment

In vitro, animal, and clinical research suggest that trimethoprim inhibits intestinal thiamine transporter ThTR-2, hepatic transporter OCT1, and renal transporters OCT2, MATE1, and MATE2, resulting in paradoxically increased thiamine plasma concentrations

Individual results may vary based on factors including age, diet, exercise, and overall health status.

They can help lighten dark spots and boost collagen when used consistently, though vitamin C is unstable and must be protected from light and air to remain effective