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These functional isoforms differ in their translation start site, resulting in length variations, with isoform A consisting of 222 amino acids and isoform X1 containing 264 amino acids, including an additional 42 amino acid segment at the N-terminus

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The last GSH synthetic process is the one depending on the conversion of the oxidized dimer GSSG to 2 reduced GSH molecules in cells by glutathione reductase (GR), an ubiquitous enzyme of the family of disulfide reductases, in the presence of NADPH and flavin adenine dinucleotide -FAD-