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The reaction mixtures were manually mixed using a 100 to 300 l multichannel pipette (set to 250 l) and the microplate was inserted into a BioTek H1 Synergy microplate reader equilibrated to 37 C and vigorously shaken for 1 min followed by a 3.5-min delay

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Blend: DSIP / Pinealon / Epitalon Strength Per Spray: 150MCG / 250MCG / 200MCG DSIP Content: 150MCG per spray Pinealon Content: 250MCG per spray Epitalon Content: 200MCG per spray Spray Count: Approximately 100 sprays Total DSIP Content: 15MG Total Pinealon Content: 25MG Total Epitalon Content: 20MG Total Peptide Content: 60MG Format: cGMP-made Aerosol research spray Research Category: Neuropeptide / pineal peptide / circadian pathway research Research Focus: Sleep-wake pathway models, CNS signaling, pineal peptide biology, circadian rhythm research, cellular aging pathway models, oxidative stress biology, and peptide-fragment response systems