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doi: 10.3390/ijms25063372 96 YangQZhengCCaoJCaoGShouPLinLet al

10.1152/ajpendo.00331.2012 202 NastasiC.FredholmS.Willerslev-OlsenA.HansenM.BonefeldC

Noteworthy, the association of GLP-1RAs and SGLT-2is is representing a new pharmacological opportunity opening an innovative scenario with potential additive pleiotropic effects that will require further studies

Subsequently, 150 L supernatant from each treatment was collected, exposed to incremental heating at 50, 55, 60, 65, 70, and 75 C for 5 min, and immediately cooled on ice to halt denaturation