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Related papers Life Sciences, 1992 ~,mrnRry The major low molecular weight thiol inside cells, the tripeptide glutathione (GSH), is of importance for protection of the cell against oxidative challenge, for thiol homeostasis required to guarantee basic functions, and for defence mechanisms against xenobiotics

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Isolated hepatocytes were plated on collagen-coated 6-well plates (4 10 4 cells per cm 2 ) in DMEM (Gibco #12800-017, containing 1 mM pyruvate, 25 mM glucose, and 4 mM glutamine) supplemented with 10 % (vol/vol) fetal bovine serum (FBS), 100 units/ml penicillin, 100 g/ml streptomycin, 1 M dexamethasone, 0.1 M insulin, and an additional 1 mM sodium pyruvate and incubated at 37 C in an atmosphere of 5 % CO 2