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Structural and functional characterization of the mutant Escherichia coli glutaredoxin (C14S) and its mixed disulfide with glutathione

Vitamin C (ascorbic acid, or AsA) was paired with glutathione in the intervention studied here because of the proposed interaction between the two molecules

This finding accounts for the important observation that the c-met binding affinity of LASSS/peroxynitrite-treated HGF (at the 1:8000:2000 molar ratio to HGF, with the wash-out of un-reacted free LASSS) was significantly higher than the original level of un-treated positive control HGF (Figure 2d, bars 2 and 6 )

This metabolite has been shown to deplete glutathione, induce oxidative stress, and cause mitochondrial injury, even in the absence of hepatic toxicity