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These are available at The following three imageJ macro scripts are required: 00-Preporcessing 01-Anisotropy Macro 03-ROI Selection The images were background-corrected using a rolling ball filter where the radius is larger than the largest cell in your images but small enough that distortions in the background are removed, in this case a radius of 100 was used

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A 29 gauge needle produces significantly less discomfort than a 25 gauge needle and is thin enough that most patients describe the sensation as a mild pinch rather than a sting