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Cell and tissue cultures Cells were cultured in Eagle's minimum essential medium supplemented with 10% (v/v) fetal bovine serum, 1% non-essential amino acids, 2 mM l-glutamine, 100 U/mL penicillin and 100 mg/mL streptomycin at 37 C in a humidified atmosphere of 5% CO 2

Candidacy is determined during a consultation

Nanda, before receiving a vitamin B12 injection treatm...ent

Results The enzyme samples are incubated in a phosphate buffer containing specific concentrations of glutathione (GSH) and peroxide and then incubated for a suitable time to react before Ellman's reagent (5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) is added