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Image analysis software was used to read the band intensity of the target proteins against the control sample after normalization by -actin ( 2.7 Histopathological analysis Hepatic injury can be detected by hematoxylin and eosin (H&E) staining of liver tissues (Mousa et al., 2022

Very recently, direct-immunofluorescence microscopy of rat lower hindlimb muscles revealed that with aging, extracellular matrix (ECM)-bound HGF undergoes tyrosine residue (Y) nitration, as visualized by anti-nitrated Y-HGF monoclonal antibodies (mAbs, rat clones 3A11C6 and 6B82C3 raised in-house) in experiments employing three age-groups of young (2-month-old), adult (10-month-old), and old rats (20-month-old) 32

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Consequently, inhibition of SLC7A11 and GPX4 expression triggers ferroptosis (47)