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Materials and methods Cell culture and reagents Rat renal proximal tubular cells (RTCs, NRK-52E) were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan), and culture cells in Dulbeccos modified Eagles medium (DMEM) were supplemented with an antibiotic/antifungal solution and 5% fetal bovine serum (FBS, pH 7.2)