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Parasite cultures were centrifuged, the pellets resuspended with 20 volumes of saponin lysis buffer (0.02% saponin, 10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 145 mM NaCl, 3 mM KCL, pH 7.2), incubated for 10 min at 37C, and washed three times with phosphate-buffered saline
Its etiopathogenesis is similar to that of actinic keratosis of the skin, and it also presents a risk of developing into squamous cell carcinoma, especially in smokers and immunosuppressed individuals
The cream is applied once a day
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